Genotyping and primer details


Genotyping and primer details for SALK_077068c - At3g62610
Line ID SALK_077068c
Locus At3g62610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23155443 of line SALK_077068c Chr3:23155443
   
Insertion allele amplicon LBb3 - 10ei
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 10ei: 5'-CATTCTCTATTGTCGTAGTTGGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23155883
   
wt amplicon (over InsPos) 03gi - 10ei
wt amplicon start Chr3:23155144
wt amplicon end Chr3:23155883
wt amplicon size 740 bp
Locus specific primer 10ei: 5'-CATTCTCTATTGTCGTAGTTGGCA-3'
2nd locus specific primer 03gi: 5'-ATTGATAATTCAGACCAAATTGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11