Genotyping and primer details


Genotyping and primer details for SALK_077395c - At5g59770
Line ID SALK_077395c
Locus At5g59770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24079903 of line SALK_077395c Chr5:24079903
   
Insertion allele amplicon R204 - CF26
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CF26: 5'-AGTGTTTTCACCAGTGTGATTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24080472
   
Insertion allele amplicon R212 - CF27
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer CF27: 5'-TTCTTTGTTTGAAAAGCTGGAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24079574
   
wt amplicon (over InsPos) CF26 - CF27
wt amplicon start Chr5:24079574
wt amplicon end Chr5:24080472
wt amplicon size 899 bp
Locus specific primer CF27: 5'-TTCTTTGTTTGAAAAGCTGGAGAT-3'
2nd locus specific primer CF26: 5'-AGTGTTTTCACCAGTGTGATTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11