Genotyping and primer details


Genotyping and primer details for SALK_082254 - At5g07240
Line ID SALK_082254
Locus At5g07240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2273149 of line SALK_082254 Chr5:2273149
   
Insertion allele amplicon LBb3 - 60ew
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 60ew: 5'-TCCAAGTGTCGACTTCTAGGATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2273599
   
wt amplicon (over InsPos) 43gc - 60ew
wt amplicon start Chr5:2272568
wt amplicon end Chr5:2273599
wt amplicon size 1032 bp
Locus specific primer 60ew: 5'-TCCAAGTGTCGACTTCTAGGATTT-3'
2nd locus specific primer 43gc: 5'-TATTTATTTGTATTGGTCCCCGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11