Genotyping and primer details


Genotyping and primer details for SALK_088702c - At2g22590
Line ID SALK_088702c
Locus At2g22590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9593760 of line SALK_088702c Chr2:9593760
   
Insertion allele amplicon LBb3 - 37dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37dr: 5'-AAAGTCTCTTTCATTTCCACTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9593138
   
wt amplicon (over InsPos) 03ev - 37dr
wt amplicon start Chr2:9593138
wt amplicon end Chr2:9594195
wt amplicon size 1058 bp
Locus specific primer 37dr: 5'-AAAGTCTCTTTCATTTCCACTCCA-3'
2nd locus specific primer 03ev: 5'-TTCAATCCTTGGTCATACACAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11