Genotyping and primer details


Genotyping and primer details for SALK_091812c - At4g24380
Line ID SALK_091812c
Locus At4g24380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12613118 of line SALK_091812c Chr4:12613118
   
Insertion allele amplicon LBb3 - 38ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38ux: 5'-GCAAGAGGGAAGACAACAGATAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12612518
   
wt amplicon (over InsPos) 73vl - 38ux
wt amplicon start Chr4:12612518
wt amplicon end Chr4:12613418
wt amplicon size 901 bp
Locus specific primer 38ux: 5'-GCAAGAGGGAAGACAACAGATAAC-3'
2nd locus specific primer 73vl: 5'-TATCAGCTGAGTTCCGTAAGGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11