Genotyping and primer details


Genotyping and primer details for SALK_093369 - At4g25390
Line ID SALK_093369
Locus At4g25390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12978222 of line SALK_093369 Chr4:12978222
   
Insertion allele amplicon LBb3 - 60qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 60qz: 5'-TCACTCTCTCCGTCTGTTTCTGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12977651
   
wt amplicon (over InsPos) 13rz - 60qz
wt amplicon start Chr4:12977651
wt amplicon end Chr4:12978567
wt amplicon size 917 bp
Locus specific primer 60qz: 5'-TCACTCTCTCCGTCTGTTTCTGTA-3'
2nd locus specific primer 13rz: 5'-TTTCACTCTCCCTCTTTCAACATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11