Genotyping and primer details


Genotyping and primer details for SALK_093745c - At5g56050
Line ID SALK_093745c
Locus At5g56050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22701169 of line SALK_093745c Chr5:22701169
   
Insertion allele amplicon LBb3 - 26ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26ty: 5'-TTGGGATTGCTACTTTGATTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22701669
   
wt amplicon (over InsPos) 19uu - 26ty
wt amplicon start Chr5:22700793
wt amplicon end Chr5:22701669
wt amplicon size 877 bp
Locus specific primer 26ty: 5'-TTGGGATTGCTACTTTGATTCTCT-3'
2nd locus specific primer 19uu: 5'-TGCTTCCTTTTGTTCTTTTGATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11