Genotyping and primer details


Genotyping and primer details for SALK_093820c - At1g22882
Line ID SALK_093820c
Locus At1g22882 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8100443 of line SALK_093820c Chr1:8100443
   
Insertion allele amplicon LBb3 - 52wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 52wm: 5'-GAGCAAAATCAGATGAACAAAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8099832
   
wt amplicon (over InsPos) 34xa - 52wm
wt amplicon start Chr1:8099832
wt amplicon end Chr1:8101077
wt amplicon size 1246 bp
Locus specific primer 52wm: 5'-GAGCAAAATCAGATGAACAAAACA-3'
2nd locus specific primer 34xa: 5'-TGAAAACCGTTAAGCATTTCTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11