Genotyping and primer details


Genotyping and primer details for SALK_094838c - At1g05210
Line ID SALK_094838c
Locus At1g05210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1511014 of line SALK_094838c Chr1:1511014
   
Insertion allele amplicon LBb3 - 10jd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 10jd: 5'-CTGAGTCAACAAACAAGAATTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1511634
   
wt amplicon (over InsPos) 14km - 10jd
wt amplicon start Chr1:1510831
wt amplicon end Chr1:1511634
wt amplicon size 804 bp
Locus specific primer 10jd: 5'-CTGAGTCAACAAACAAGAATTCCA-3'
2nd locus specific primer 14km: 5'-TATTGGTCTGAACTTGGGAGACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11