Genotyping and primer details


Genotyping and primer details for SALK_096324c - At4g29360
Line ID SALK_096324c
Locus At4g29360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14453292 of line SALK_096324c Chr4:14453292
   
Insertion allele amplicon LBb3 - 91ns
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 91ns: 5'-GTAGTACTCTTTGCACTCGAACCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14454092
   
wt amplicon (over InsPos) 13or - 91ns
wt amplicon start Chr4:14453078
wt amplicon end Chr4:14454092
wt amplicon size 1015 bp
Locus specific primer 91ns: 5'-GTAGTACTCTTTGCACTCGAACCC-3'
2nd locus specific primer 13or: 5'-AATCGAAGTGATTTTGGTAGATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11