Genotyping and primer details


Genotyping and primer details for SALK_096772c - At4g35360
Line ID SALK_096772c
Locus At4g35360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16812636 of line SALK_096772c Chr4:16812636
   
Insertion allele amplicon LBb3 - 92ns
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 92ns: 5'-GGTGATGAATAGAGTCATTTGGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16812959
   
wt amplicon (over InsPos) 04ph - 92ns
wt amplicon start Chr4:16812132
wt amplicon end Chr4:16812959
wt amplicon size 828 bp
Locus specific primer 92ns: 5'-GGTGATGAATAGAGTCATTTGGGT-3'
2nd locus specific primer 04ph: 5'-ATCAGAAAGATTGGTGCTTTTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11