Genotyping and primer details


Genotyping and primer details for SALK_098437c - At2g33830
Line ID SALK_098437c
Locus At2g33830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14310333 of line SALK_098437c Chr2:14310333
   
Insertion allele amplicon LBb3 - 62qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 62qz: 5'-CTAAAAACTAACCCAGATGGTGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14309599
   
wt amplicon (over InsPos) 37rz - 62qz
wt amplicon start Chr2:14309599
wt amplicon end Chr2:14310724
wt amplicon size 1126 bp
Locus specific primer 62qz: 5'-CTAAAAACTAACCCAGATGGTGGA-3'
2nd locus specific primer 37rz: 5'-CTAATTTCCAAAGATGGCGTGTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11