Genotyping and primer details


Genotyping and primer details for SALK_098460c - At4g19660
Line ID SALK_098460c
Locus At4g19660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10697358 of line SALK_098460c Chr4:10697358
   
Insertion allele amplicon LBb3 - 44cp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 44cp: 5'-TCATACGTGTAAGCATTCTTTTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10696625
   
wt amplicon (over InsPos) 31lt - 44cp
wt amplicon start Chr4:10696625
wt amplicon end Chr4:10697738
wt amplicon size 1114 bp
Locus specific primer 44cp: 5'-TCATACGTGTAAGCATTCTTTTCG-3'
2nd locus specific primer 31lt: 5'-TTTCGTCATTTCAGGTTTGAGATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11