Genotyping and primer details


Genotyping and primer details for SALK_099763c - At4g32130
Line ID SALK_099763c
Locus At4g32130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15520601 of line SALK_099763c Chr4:15520601
   
Insertion allele amplicon LBb3 - 25kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25kh: 5'-ATAAACCAATGTTAGATCGCTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15520224
   
wt amplicon (over InsPos) 09mc - 25kh
wt amplicon start Chr4:15520224
wt amplicon end Chr4:15521131
wt amplicon size 908 bp
Locus specific primer 25kh: 5'-ATAAACCAATGTTAGATCGCTTGG-3'
2nd locus specific primer 09mc: 5'-GAAGTTTATGCACTGGGCTACTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11