Genotyping and primer details


Genotyping and primer details for SALK_099815c - At4g21310
Line ID SALK_099815c
Locus At4g21310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11339601 of line SALK_099815c Chr4:11339601
   
Insertion allele amplicon LBb3 - 33mv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33mv: 5'-AGAAAACAAATCTTTTCGAGGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11340110
   
wt amplicon (over InsPos) 10oj - 33mv
wt amplicon start Chr4:11339166
wt amplicon end Chr4:11340110
wt amplicon size 945 bp
Locus specific primer 33mv: 5'-AGAAAACAAATCTTTTCGAGGACA-3'
2nd locus specific primer 10oj: 5'-TTGAAGCAGTTGCAGAGATGTAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11