Genotyping and primer details


Genotyping and primer details for SALK_099974 - At4g02200
Line ID SALK_099974
Locus At4g02200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:974167 of line SALK_099974 Chr4:974167
   
Insertion allele amplicon LBb3 - 44wj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 44wj: 5'-ATCGATGAGCCGTTGTCTACTAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:973824
   
wt amplicon (over InsPos) 09wy - 44wj
wt amplicon start Chr4:973824
wt amplicon end Chr4:974706
wt amplicon size 883 bp
Locus specific primer 44wj: 5'-ATCGATGAGCCGTTGTCTACTAAT-3'
2nd locus specific primer 09wy: 5'-AACGAATGCTTTGTAGCTATGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11