Genotyping and primer details


Genotyping and primer details for SALK_100384c - At4g29860
Line ID SALK_100384c
Locus At4g29860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14603030 of line SALK_100384c Chr4:14603030
   
Insertion allele amplicon LBb3 - 53vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 53vi: 5'-ATCCAAATCTCCAGAAAACAGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14602388
   
wt amplicon (over InsPos) 60vw - 53vi
wt amplicon start Chr4:14602388
wt amplicon end Chr4:14603393
wt amplicon size 1006 bp
Locus specific primer 53vi: 5'-ATCCAAATCTCCAGAAAACAGAGA-3'
2nd locus specific primer 60vw: 5'-CTGTGTCCTACTCTCCAGACTGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11