Genotyping and primer details


Genotyping and primer details for SALK_101062c - At2g23130
Line ID SALK_101062c
Locus At2g23130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9844852 of line SALK_101062c Chr2:9844852
   
Insertion allele amplicon LBb3 - 04gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 04gq: 5'-AGAACAAGTAGAGACCATGCGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9845176
   
wt amplicon (over InsPos) 13kj - 04gq
wt amplicon start Chr2:9844425
wt amplicon end Chr2:9845176
wt amplicon size 752 bp
Locus specific primer 04gq: 5'-AGAACAAGTAGAGACCATGCGATT-3'
2nd locus specific primer 13kj: 5'-TCTCTTGACGGTTACATTGATCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11