Genotyping and primer details


Genotyping and primer details for SALK_104317c - At1g52770
Line ID SALK_104317c
Locus At1g52770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19656572 of line SALK_104317c Chr1:19656572
   
Insertion allele amplicon LBb3 - 93ns
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 93ns: 5'-ATTCTTGGCATTGATTATTTCGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19656108
   
wt amplicon (over InsPos) 03oy - 93ns
wt amplicon start Chr1:19656108
wt amplicon end Chr1:19657191
wt amplicon size 1084 bp
Locus specific primer 93ns: 5'-ATTCTTGGCATTGATTATTTCGTC-3'
2nd locus specific primer 03oy: 5'-CCAGAATCTGAGTAGTGTGAACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11