Genotyping and primer details


Genotyping and primer details for SALK_105351c - At1g76140
Line ID SALK_105351c
Locus At1g76140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28573275 of line SALK_105351c Chr1:28573275
   
Insertion allele amplicon LBb3 - 53dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 53dh: 5'-GAGAAGGAGAGGATATTGATGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28572479
   
wt amplicon (over InsPos) 42gc - 53dh
wt amplicon start Chr1:28572479
wt amplicon end Chr1:28573462
wt amplicon size 984 bp
Locus specific primer 53dh: 5'-GAGAAGGAGAGGATATTGATGCTG-3'
2nd locus specific primer 42gc: 5'-AGAGAGTGTGTTACCTGAATGGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11