Genotyping and primer details


Genotyping and primer details for SALK_107824c - At5g15450
Line ID SALK_107824c
Locus At5g15450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5017437 of line SALK_107824c Chr5:5017437
   
Insertion allele amplicon LBb3 - 31gd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31gd: 5'-ACAATCAAAGGAAATTTGCATACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5016709
   
wt amplicon (over InsPos) 47et - 31gd
wt amplicon start Chr5:5016709
wt amplicon end Chr5:5017574
wt amplicon size 866 bp
Locus specific primer 31gd: 5'-ACAATCAAAGGAAATTTGCATACC-3'
2nd locus specific primer 47et: 5'-TGTTCCAGAGAGCAAGACAGTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11