Genotyping and primer details


Genotyping and primer details for SALK_108755c - At2g36620
Line ID SALK_108755c
Locus At2g36620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15351814 of line SALK_108755c Chr2:15351814
   
Insertion allele amplicon LBb3 - 13mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13mh: 5'-GATTTGAAACAGAGACCAAACACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15351509
   
wt amplicon (over InsPos) 07ng - 13mh
wt amplicon start Chr2:15351509
wt amplicon end Chr2:15352204
wt amplicon size 696 bp
Locus specific primer 13mh: 5'-GATTTGAAACAGAGACCAAACACA-3'
2nd locus specific primer 07ng: 5'-CCATATACCATTTTAGCCGTGAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11