Genotyping and primer details


Genotyping and primer details for SALK_109663 - At4g34090
Line ID SALK_109663
Locus At4g34090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16328094 of line SALK_109663 Chr4:16328094
   
Insertion allele amplicon LBb3 - 46um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46um: 5'-GACATTAGGATGTCCGTATGAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16328419
   
wt amplicon (over InsPos) 59vr - 46um
wt amplicon start Chr4:16327450
wt amplicon end Chr4:16328419
wt amplicon size 970 bp
Locus specific primer 46um: 5'-GACATTAGGATGTCCGTATGAGGT-3'
2nd locus specific primer 59vr: 5'-TACGAAGCTGCTGCTAAAGTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11