Genotyping and primer details


Genotyping and primer details for SALK_110091 - At4g33010
Line ID SALK_110091
Locus At4g33010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15927185 of line SALK_110091 Chr4:15927185
   
Insertion allele amplicon R204 - CE49
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CE49: 5'-TGAGAGGCTTCAAGGTATGAAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15927664
   
Insertion allele amplicon R212 - CE50
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer CE50: 5'-GAAGCATTTTTCTTTTCATCTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15926612
   
wt amplicon (over InsPos) CE49 - CE50
wt amplicon start Chr4:15926612
wt amplicon end Chr4:15927664
wt amplicon size 1053 bp
Locus specific primer CE50: 5'-GAAGCATTTTTCTTTTCATCTTGC-3'
2nd locus specific primer CE49: 5'-TGAGAGGCTTCAAGGTATGAAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11