Genotyping and primer details


Genotyping and primer details for SALK_110849 - At1g13830
Line ID SALK_110849
Locus At1g13830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4740639 of line SALK_110849 Chr1:4740639
   
Insertion allele amplicon LBb3 - 54wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 54wm: 5'-TCCACCTTTCATTTTCCAATACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4741058
   
wt amplicon (over InsPos) 33xa - 54wm
wt amplicon start Chr1:4740059
wt amplicon end Chr1:4741058
wt amplicon size 1000 bp
Locus specific primer 54wm: 5'-TCCACCTTTCATTTTCCAATACTC-3'
2nd locus specific primer 33xa: 5'-ACAGAGTGTGAAAACAGAGGAGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11