Genotyping and primer details


Genotyping and primer details for SALK_111366 - At3g56140
Line ID SALK_111366
Locus At3g56140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20830295 of line SALK_111366 Chr3:20830295
   
Insertion allele amplicon LBb3 - 29ah
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29ah: 5'-AACTCGAAATCGTTAGAAACCTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20829942
   
Insertion allele amplicon R204 - AD05
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AD05: 5'-CCTGTCACCACTAACAGTAGACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20830864
   
wt amplicon (over InsPos) 29ah - AD05
wt amplicon start Chr3:20829942
wt amplicon end Chr3:20830864
wt amplicon size 923 bp
Locus specific primer AD05: 5'-CCTGTCACCACTAACAGTAGACCA-3'
2nd locus specific primer 29ah: 5'-AACTCGAAATCGTTAGAAACCTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11