Genotyping and primer details


Genotyping and primer details for SALK_111655c - At4g29870
Line ID SALK_111655c
Locus At4g29870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14606358 of line SALK_111655c Chr4:14606358
   
Insertion allele amplicon LBb3 - 26kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26kh: 5'-CTTAGTCAACAACACCTGAAGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14606828
   
wt amplicon (over InsPos) 04mc - 26kh
wt amplicon start Chr4:14605813
wt amplicon end Chr4:14606828
wt amplicon size 1016 bp
Locus specific primer 26kh: 5'-CTTAGTCAACAACACCTGAAGCAA-3'
2nd locus specific primer 04mc: 5'-AAGAGAAAGCCTTTGAAACTCTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11