Genotyping and primer details


Genotyping and primer details for SALK_112870 - At1g76390
Line ID SALK_112870
Locus At1g76390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28657006 of line SALK_112870 Chr1:28657006
   
Insertion allele amplicon LBb3 - 55cy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 55cy: 5'-ACGGTCAACCTCTATCTTGTCCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28656107
   
wt amplicon (over InsPos) 30lt - 55cy
wt amplicon start Chr1:28656107
wt amplicon end Chr1:28657413
wt amplicon size 1307 bp
Locus specific primer 55cy: 5'-ACGGTCAACCTCTATCTTGTCCTA-3'
2nd locus specific primer 30lt: 5'-GACAGCTAGTGAGTCCAACAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11