Genotyping and primer details


Genotyping and primer details for SALK_114083c - At1g08410
Line ID SALK_114083c
Locus At1g08410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2648519 of line SALK_114083c Chr1:2648519
   
Insertion allele amplicon LBb3 - 07cw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07cw: 5'-CCAATCCTCTTGTGCCTTAATACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2647906
   
wt amplicon (over InsPos) 13da - 07cw
wt amplicon start Chr1:2647906
wt amplicon end Chr1:2648720
wt amplicon size 815 bp
Locus specific primer 07cw: 5'-CCAATCCTCTTGTGCCTTAATACT-3'
2nd locus specific primer 13da: 5'-TGTAATCTTTCAGAATCAGCCTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11