Genotyping and primer details


Genotyping and primer details for SALK_115705c - At2g45150
Line ID SALK_115705c
Locus At2g45150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18613750 of line SALK_115705c Chr2:18613750
   
Insertion allele amplicon LBb3 - 95ns
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 95ns: 5'-ACAGTGTAAGTATGGGCATAAGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18614229
   
wt amplicon (over InsPos) 11or - 95ns
wt amplicon start Chr2:18613396
wt amplicon end Chr2:18614229
wt amplicon size 834 bp
Locus specific primer 95ns: 5'-ACAGTGTAAGTATGGGCATAAGGG-3'
2nd locus specific primer 11or: 5'-AACCAAATGACAAATACGAACACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11