Genotyping and primer details


Genotyping and primer details for SALK_116999c - At4g02730
Line ID SALK_116999c
Locus At4g02730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1207778 of line SALK_116999c Chr4:1207778
   
Insertion allele amplicon LBb3 - 90ns
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 90ns: 5'-CATTCATAAGGAGATCTAGCGTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1208117
   
wt amplicon (over InsPos) 04px - 90ns
wt amplicon start Chr4:1207322
wt amplicon end Chr4:1208117
wt amplicon size 796 bp
Locus specific primer 90ns: 5'-CATTCATAAGGAGATCTAGCGTCC-3'
2nd locus specific primer 04px: 5'-TCTTGCATAAATCTTCCCTTCTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11