Genotyping and primer details


Genotyping and primer details for SALK_117268 - At4g37450
Line ID SALK_117268
Locus At4g37450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17605932 of line SALK_117268 Chr4:17605932
   
Insertion allele amplicon LBb3 - 21gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 21gq: 5'-TCTCACTTTCTTTTTGCCTACCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17605164
   
wt amplicon (over InsPos) 15hs - 21gq
wt amplicon start Chr4:17605164
wt amplicon end Chr4:17606152
wt amplicon size 989 bp
Locus specific primer 21gq: 5'-TCTCACTTTCTTTTTGCCTACCAT-3'
2nd locus specific primer 15hs: 5'-CGTCACAAGTTCGAGTATCAATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11