Genotyping and primer details


Genotyping and primer details for SALK_117497c - At4g26600
Line ID SALK_117497c
Locus At4g26600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13420866 of line SALK_117497c Chr4:13420866
   
Insertion allele amplicon LBb3 - 12oz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12oz: 5'-ACGAGAGGAATGATTATGTTGGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13420509
   
wt amplicon (over InsPos) 03px - 12oz
wt amplicon start Chr4:13420509
wt amplicon end Chr4:13421432
wt amplicon size 924 bp
Locus specific primer 12oz: 5'-ACGAGAGGAATGATTATGTTGGAC-3'
2nd locus specific primer 03px: 5'-TTGACAGCACTTATCATCACAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11