Genotyping and primer details


Genotyping and primer details for SALK_118230c - At1g02390
Line ID SALK_118230c
Locus At1g02390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:481342 of line SALK_118230c Chr1:481342
   
Insertion allele amplicon LBb3 - 16jg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16jg: 5'-AATTACAATCATGCAAGCTGTACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:480718
   
wt amplicon (over InsPos) 16km - 16jg
wt amplicon start Chr1:480718
wt amplicon end Chr1:481506
wt amplicon size 789 bp
Locus specific primer 16jg: 5'-AATTACAATCATGCAAGCTGTACG-3'
2nd locus specific primer 16km: 5'-TTTCAAGGTTCTTCTTATCCTCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11