Genotyping and primer details


Genotyping and primer details for SALK_118506c - At4g13660
Line ID SALK_118506c
Locus At4g13660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7948263 of line SALK_118506c Chr4:7948263
   
Insertion allele amplicon LBb3 - 40cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 40cv: 5'-TGGAACGCTCTCTCTTCATATTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7947496
   
wt amplicon (over InsPos) 26dz - 40cv
wt amplicon start Chr4:7947496
wt amplicon end Chr4:7948423
wt amplicon size 928 bp
Locus specific primer 40cv: 5'-TGGAACGCTCTCTCTTCATATTTT-3'
2nd locus specific primer 26dz: 5'-TTTTCGGACCCTTGAGATTATATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11