Genotyping and primer details


Genotyping and primer details for SALK_118557c - At4g11300
Line ID SALK_118557c
Locus At4g11300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:6872419 of line SALK_118557c Chr4:6872419
   
Insertion allele amplicon LBb3 - 24ih
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 24ih: 5'-ATGGTGATGTGGACGTTAGTTGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6872734
   
wt amplicon (over InsPos) 11kj - 24ih
wt amplicon start Chr4:6872107
wt amplicon end Chr4:6872734
wt amplicon size 628 bp
Locus specific primer 24ih: 5'-ATGGTGATGTGGACGTTAGTTGTA-3'
2nd locus specific primer 11kj: 5'-AATTAGAACATCAAAATGGCTTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11