Genotyping and primer details


Genotyping and primer details for SALK_118898 - At1g22330
Line ID SALK_118898
Locus At1g22330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7888735 of line SALK_118898 Chr1:7888735
   
Insertion allele amplicon LBb3 - 20vt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20vt: 5'-GATTGTACAAGTTGAGGCAGTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7889265
   
wt amplicon (over InsPos) 22wq - 20vt
wt amplicon start Chr1:7888143
wt amplicon end Chr1:7889265
wt amplicon size 1123 bp
Locus specific primer 20vt: 5'-GATTGTACAAGTTGAGGCAGTTGA-3'
2nd locus specific primer 22wq: 5'-CTTCTAGCGTATGTGAATGTTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11