Genotyping and primer details


Genotyping and primer details for SALK_119243c - At4g01190
Line ID SALK_119243c
Locus At4g01190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:505641 of line SALK_119243c Chr4:505641
   
Insertion allele amplicon LBb3 - 25ih
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25ih: 5'-TCAATCACTGAGTTTGATTGGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:506059
   
wt amplicon (over InsPos) 03km - 25ih
wt amplicon start Chr4:505194
wt amplicon end Chr4:506059
wt amplicon size 866 bp
Locus specific primer 25ih: 5'-TCAATCACTGAGTTTGATTGGAAG-3'
2nd locus specific primer 03km: 5'-GTAATGAGATGACCCCTTTTGTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11