Genotyping and primer details


Genotyping and primer details for SALK_120081c - At3g56420
Line ID SALK_120081c
Locus At3g56420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20922154 of line SALK_120081c Chr3:20922154
   
Insertion allele amplicon LBb3 - 56wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 56wm: 5'-AATCGAGAAATGGGAAGAAAAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20922651
   
wt amplicon (over InsPos) 32xa - 56wm
wt amplicon start Chr3:20921383
wt amplicon end Chr3:20922651
wt amplicon size 1269 bp
Locus specific primer 56wm: 5'-AATCGAGAAATGGGAAGAAAAGAT-3'
2nd locus specific primer 32xa: 5'-AGTAGTCACCATTGGATTTGGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11