Genotyping and primer details


Genotyping and primer details for SALK_120653c - At4g08170
Line ID SALK_120653c
Locus At4g08170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:5165415 of line SALK_120653c Chr4:5165415
   
Insertion allele amplicon LBb3 - 26ih
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26ih: 5'-GTCTGTCATTCTGTGGTGATGAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5165974
   
wt amplicon (over InsPos) 09kj - 26ih
wt amplicon start Chr4:5165051
wt amplicon end Chr4:5165974
wt amplicon size 924 bp
Locus specific primer 26ih: 5'-GTCTGTCATTCTGTGGTGATGAGT-3'
2nd locus specific primer 09kj: 5'-ATGTGCTAGCCTAAATTCCTGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11