Genotyping and primer details


Genotyping and primer details for SALK_120706 - At2g25000
Line ID SALK_120706
Locus At2g25000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10629829 of line SALK_120706 Chr2:10629829
   
Insertion allele amplicon LBb3 - 15ke
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15ke: 5'-TGATTTGTTCAACTTTTGAGCATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10630178
   
wt amplicon (over InsPos) 09ls - 15ke
wt amplicon start Chr2:10629248
wt amplicon end Chr2:10630178
wt amplicon size 931 bp
Locus specific primer 15ke: 5'-TGATTTGTTCAACTTTTGAGCATC-3'
2nd locus specific primer 09ls: 5'-GCCAACAAATTTAATCCAACGTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11