Genotyping and primer details


Genotyping and primer details for SALK_121735c - At1g13960
Line ID SALK_121735c
Locus At1g13960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4776734 of line SALK_121735c Chr1:4776734
   
Insertion allele amplicon LBb3 - 29dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29dh: 5'-CCTTTTGTTCTGTCTGAAAGTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4777420
   
wt amplicon (over InsPos) 27dz - 29dh
wt amplicon start Chr1:4776294
wt amplicon end Chr1:4777420
wt amplicon size 1127 bp
Locus specific primer 29dh: 5'-CCTTTTGTTCTGTCTGAAAGTTCC-3'
2nd locus specific primer 27dz: 5'-ATATATCTGCAGCGTGGACTTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11