Genotyping and primer details


Genotyping and primer details for SALK_122023c - At2g01800
Line ID SALK_122023c
Locus At2g01800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:344607 of line SALK_122023c Chr2:344607
   
Insertion allele amplicon LBb3 - BI10
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BI10: 5'-ATAGCTTCCGGAGTAGCATTAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:344040
   
wt amplicon (over InsPos) 12xf - BI10
wt amplicon start Chr2:344040
wt amplicon end Chr2:345781
wt amplicon size 1742 bp
Locus specific primer BI10: 5'-ATAGCTTCCGGAGTAGCATTAACA-3'
2nd locus specific primer 12xf: 5'-CATATCACACTAAGATGGGCTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11