Genotyping and primer details


Genotyping and primer details for SALK_122435c - At3g49840
Line ID SALK_122435c
Locus At3g49840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18485521 of line SALK_122435c Chr3:18485521
   
Insertion allele amplicon LBb3 - 63vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 63vi: 5'-ACAAAGAAGACTTGAGCACATTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18484942
   
wt amplicon (over InsPos) 74vw - 63vi
wt amplicon start Chr3:18484942
wt amplicon end Chr3:18486284
wt amplicon size 1343 bp
Locus specific primer 63vi: 5'-ACAAAGAAGACTTGAGCACATTGA-3'
2nd locus specific primer 74vw: 5'-CAACTCTCAGTTTCATCATCTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11