Genotyping and primer details


Genotyping and primer details for SALK_122458 - At5g65750
Line ID SALK_122458
Locus At5g65750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26307915 of line SALK_122458 Chr5:26307915
   
Insertion allele amplicon LBb3 - 45kk
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45kk: 5'-TCAGGTCTACTATGAGCTTGACGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26307494
   
wt amplicon (over InsPos) 05lz - 45kk
wt amplicon start Chr5:26307494
wt amplicon end Chr5:26308379
wt amplicon size 886 bp
Locus specific primer 45kk: 5'-TCAGGTCTACTATGAGCTTGACGA-3'
2nd locus specific primer 05lz: 5'-AAAGCTTGGAAGAAGACAGTGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11