Genotyping and primer details


Genotyping and primer details for SALK_125066c - At1g30630
Line ID SALK_125066c
Locus At1g30630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10858779 of line SALK_125066c Chr1:10858779
   
Insertion allele amplicon LBb3 - 03du
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 03du: 5'-AACAGATTGATGAAGACCACACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10859320
   
wt amplicon (over InsPos) 09fg - 03du
wt amplicon start Chr1:10858225
wt amplicon end Chr1:10859320
wt amplicon size 1096 bp
Locus specific primer 03du: 5'-AACAGATTGATGAAGACCACACAC-3'
2nd locus specific primer 09fg: 5'-AGCATTCCTGGCTTTTAATTTGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11