Genotyping and primer details


Genotyping and primer details for SALK_126799c - At3g04920
Line ID SALK_126799c
Locus At3g04920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1361742 of line SALK_126799c Chr3:1361742
   
Insertion allele amplicon LBb3 - 26rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26rb: 5'-TGTTTTAGGTCAGGCTTTCAGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1361215
   
wt amplicon (over InsPos) 07se - 26rb
wt amplicon start Chr3:1361215
wt amplicon end Chr3:1361954
wt amplicon size 740 bp
Locus specific primer 26rb: 5'-TGTTTTAGGTCAGGCTTTCAGATT-3'
2nd locus specific primer 07se: 5'-CATACCTTCTTAACACCACGGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11