Genotyping and primer details


Genotyping and primer details for SALK_127086c - At5g09690
Line ID SALK_127086c
Locus At5g09690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3001812 of line SALK_127086c Chr5:3001812
   
Insertion allele amplicon LBb3 - 47kq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 47kq: 5'-TGCAAGGACAACAGAACTAGAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3002328
   
wt amplicon (over InsPos) 04oj - 47kq
wt amplicon start Chr5:3001384
wt amplicon end Chr5:3002328
wt amplicon size 945 bp
Locus specific primer 47kq: 5'-TGCAAGGACAACAGAACTAGAGAA-3'
2nd locus specific primer 04oj: 5'-TGATTTCGATGATTGTCTAACTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11