Genotyping and primer details


Genotyping and primer details for SALK_128430c - At1g06760
Line ID SALK_128430c
Locus At1g06760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2077539 of line SALK_128430c Chr1:2077539
   
Insertion allele amplicon LBb3 - 19cl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19cl: 5'-ATTTAAGGGGTAGTCCAAAGGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2076551
   
wt amplicon (over InsPos) 24lt - 19cl
wt amplicon start Chr1:2076551
wt amplicon end Chr1:2077931
wt amplicon size 1381 bp
Locus specific primer 19cl: 5'-ATTTAAGGGGTAGTCCAAAGGAAC-3'
2nd locus specific primer 24lt: 5'-AAAGCACAACAAATATGACCAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11