Genotyping and primer details


Genotyping and primer details for SALK_129236c - At1g05340
Line ID SALK_129236c
Locus At1g05340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1558883 of line SALK_129236c Chr1:1558883
   
Insertion allele amplicon LBb3 - SC03
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer SC03: 5'-ATGTCTACCTGTACATCACCACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1559497
   
wt amplicon (over InsPos) 04cd - SC03
wt amplicon start Chr1:1558652
wt amplicon end Chr1:1559497
wt amplicon size 846 bp
Locus specific primer SC03: 5'-ATGTCTACCTGTACATCACCACCA-3'
2nd locus specific primer 04cd: 5'-ATTTGGATTTGTCTTTTCAATTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11